Journal: Bone & Joint Research

Article Title: Genotype and phenotype correlation analysis in 27 families with multiple osteochondroma and validation by ATDC5 chondrocyte models

doi: 10.1302/2046-3758.1410.BJR-2024-0477.R1

Figure Lengend Snippet: The evaluation of chondrocyte differentiation. The expression of differentiation markers of chondrocytes (α-1 chain of type X collagen ( COL10A1 ), matrix metallopeptidase 13 ( MMP13 ), and Runt-related transcription factor 2 ( RUNX2 )), as measured by a) to c) quantitative real-time polymerase chain reaction (qRT-PCR) and d) western blot, was significantly higher in the EXT1 -/- and EXT2 -/- groups compared with the wild type (WT) group. e) and f) The results of Alizarin red staining (magnification: 10×) on day 21 also showed that calcium deposition (stained red) was significantly increased in the EXT1 -/- and EXT2 -/- groups compared with the WT group. *p < 0.05, **p < 0.01; independent-samples t -test. Scale bar = 200 μm. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; KD, knockdown; mRNA, messenger RNA; ns, no significance.

Article Snippet: Membranes were probed with primary antibodies as follows: EXT1 (1:1,000 dilution, Thermo Fisher Scientific), EXT2 (1:1,000 dilution, Proteintech, USA), Aggrecan Monoclonal Antibody (BC-3) (ACAN) (1:1,000 dilution, Thermo Fisher Scientific), α-1 chain of type I collagen (COL1A1) (1:1,000 dilution, Cell Signaling Technology (CST), USA), SOX9 (1:5,000 dilution, Proteintech), RUNX2 (1:2,000 dilution, Proteintech), α-1 chain of type X collagen (COL10A1) (1:1,000 dilution, Proteintech), MMP13 (1:1,000 dilution, Proteintech), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10,000 dilution, Proteintech), and Tubulin (1:5,000 dilution, Proteintech).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Staining, Knockdown

Journal: American Journal of Physiology - Renal Physiology

Article Title: The mechanosensitive BKα/β1 channel localizes to cilia of principal cells in rabbit cortical collecting duct (CCD)

doi: 10.1152/ajprenal.00256.2016

Figure Lengend Snippet: Antibodies (Abs) used for immunoperfusion

Article Snippet: 3D reconstructions were generated using Leica Application Suite (LAS AF) software. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Primary Abs Dilution Provider Secondary Abs Dilution Provider Goat IgG anti-maxi-Kβ1 (N-15) 1:100 Santa Cruz Biotechnology (sc-14749) A488-rabbit IgG anti-goat IgG 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A11078","term_id":"490929","term_text":"A11078"}} A11078 ) Mouse IgG2a anti-maxi-Kβ4 1:100 Stress Marq Biosciences (SMC-38D) A488-goat IgG anti-mouse IgG2a 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A21131","term_id":"514092","term_text":"A21131"}} A21131 ) Mouse IgG2b anti-acetylated α-tubulin (6-11B-1) 1:1,000 Abcam (ab24610) A488-goat IgG anti-mouse IgG 1:1,000 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A11029","term_id":"492395","term_text":"A11029"}} A11029 ) Goat IgG anti-aquaporin-2 (C-17) 1:100 Santa Cruz Biotechnology (sc-9882) A488-rabbit IgG anti-goat IgG 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A11078","term_id":"490929","term_text":"A11078"}} A11078 ) Mouse IgG2a anti-polycystin-2 (YCE2) 1:100 Santa Cruz Biotechnology (sc-47734) A647-goat IgG anti-mouse IgG 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A21236","term_id":"583506","term_text":"A21236"}} A21236 ) Rabbit anti-pendrin 1:2,000 Gift from Dr. S. Wall, Emory University School of Medicine A488-goat IgG anti-rabbit IgG 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A11008","term_id":"492390","term_text":"A11008"}} A11008 ) Open in a separate window Antibodies (Abs) used for immunoperfusion The effect of K + intake on relative BKα expression in the cilia was determined by analysis of CCDs isolated from HK ( n = 4)- or LK ( n = 4)-fed rabbits and immunoperfused simultaneously with Abs directed against BKα and acetylated α-tubulin (Ac α-tubulin), followed by appropriate secondary Abs ( ).

Techniques: